Skip to content

Isotopes and Isotope Batch

Isotope

Isotopes can be an important step in the identification process of missing persons. If your project chooses to use Isotope analysis as part of your identification process then you have come to the right place. Here you will learn more about the Isotope module in CoRA. A critical first step is to cut a sample of the specimen for Isotope analysis and then assign a sample number to it for tracking purposes and after you get results from your Isotope lab you can capture Isotope information here to create a Isotope Profile for further analysis.

Create a Isotope Sample

The Isotope sample and Profile are associated with a specimen, so you must first choose a specimen for which you want to create a Isotope sample for. You can choose a specimen by navigating to the specimen screen from either the specimen search results screen or from any data table row that has a specimen link on it. All search and reports screens have specimen links on them. Simply click these links to navigate to the specimen view/edit screen. Once on the specimen screen you can navigate to the Specimen Action or Details, and then to the Isotope Profile menu.

CoRA's intellisense will automatically take you to the Create Isotope sample screen, if this is the first Isotope sample you are trying to create for this specimen or will take you to the Isotope list screen if a Isotope Sample was previously created for this specimen. Note you can create multiple Isotope samples for a specimen.

Tip

CoRA allows you to create multiple Isotope samples for a specimen. If you have multiple isotope samples, CoRA will create a Consensus Isotope Profile for you with data from the multiple samples. Consensus Isotope Profile is a powerful feature of CoRA.

The screen fields are shown below. Fields in bold are required.

Attributes Data Type Size Validation Rule Required/Optional
Sample Number Alphanumeric 8 chars can only contain letters and numbers Required
Lab Id id valid labs via dropdown setup for the org Required
External Case Number String 32 chars Optional

Clicking Save will take you to the Isotope Profile screen. You can visit the Isotope Profile screen once you have received data from your Isotope processing lab to enter Isotope related data.


Edit Isotope Profile

Specimens which have had a Isotope sample successfully created will have an associated Isotope Profile. The Isotope profile contains all the relevant Isotope attributes and are editable via action (user must have appropriate role and permissions).

You can visit the Isotope Profile screen once you have received data from your Isotope processing lab to enter Isotope related data to build the Isotope Profile for the specimen. On this screen click the pencil icon on the top right corner to edit the Isotope profile.

The screen fields are shown below. Fields in bold are required.

Attributes Data Type Size Validation Rule Required/Optional
Sample Number Alphanumeric 8 chars can only contain letters and numbers Required
Lab Id id valid labs via dropdown setup for the org Required
External Case Number String 32 chars Optional
Isotope Batch Number Number valid numeric value Optional
Results Status String 32 chars valid results status via dropdown Optional
Sample Cleaned Weight Decimal Optional
Demineraliing Start Date Date Optional
Demineraliing End Date Date Optional
Sample Vial and Lid Weight Decimal Optional
Weight Collagen Decimal Optional
Yield Collagen Decimal Optional
Collagen Weight used for Analysis Decimal Optional
Analysis Requested String Optional
Well Location String Optional
Carbon Delta Decimal Optional
Carbon Weight Decimal Optional
Carbon Percentage Decimal Optional
Nitrogen Delta Decimal Optional
Nitrogen Weight Decimal Optional
Nitrogen Percentage Decimal Optional
Carbon/Nitrogen Ratio Decimal Optional
Oxygen Delta Decimal Optional
Oxygen Weight Decimal Optional
Oxygen Percentage Decimal Optional
Carbon/Oxygen Ratio Decimal Optional
Sulphur Delta Decimal Optional
Sulphur Weight Decimal Optional
Sulphur Percentage Decimal Optional

Click on Save if any of the fields were updated

Tip

The Isotope profile has attributes to track Carbon, Nitrogen, Oxygen, Sulphur and respective deltas, weight, percentage and relevant ratios for further analysis.

Isotope Batch

A new isotope batch can be created by navigating to Left Sidebar Menu -> Isotope -> New Isotope Batch.

This will allow an Isotope Analyst to first create an isotope batch with a batch number and then associate specimen isotope samples that belong to that batch. After all the isotopes samples are added to the batch we can start processing the isotope batch. The isotope batch will step through multiple processing steps which as detailed below.

We can track the progress throughout the processing cycle of an isotope batch. This functionality will allow the Isotope Analyst digitally document at which step the Isotope Batch is currently in and tracks its' progress and status.

Tip

You must be logged in as an Isotope Analyst in order to create a new isotope batch.

The screen fields are shown below. Fields in bold are required.

Attributes Data Type Size Validation Rule Required/Optional
Batch Number Alphanumeric 8 chars can only contain letters and numbers Required
Lab Id id valid labs via dropdown setup for the org Required
External Case Number String 32 chars Optional

Clicking Save will create the isotope batch in the database and will allow you to now associate isotope samples to this batch

Associate Isotopes Samples

Once an isotope batch has been created the user will be allowed to associate isotope samples to this batch. In order to associate isotope samples to this batch the user must first select the project, this will then show you all the specimen isotope samples that are available to be added to this batch. Selecting the isotope sample and clicking the associate button will move the sample into the associated isotopes list, meaning that the sample has now been added to this batch.

The screen fields are shown below. Fields in bold are required.

Attributes Data Type Size Validation Rule Required/Optional
Project Id id valid projects via dropdown setup for the org Required
Isotopes Sample String 32 chars Required
Associated Samples String 32 chars Required

Note

An isotope batch must have at least one isotope sample associated with it.

Start Batch Processing

The start processing button will be enable once isotope samples are associated with the isotope batch. The batch processing has multiple steps and the following sections describe each of these steps in the order they are to be performed. You cannot move forward unless all the tasks in a given step are completed, you can move back to look the data in the previous steps. Only when all steps have been completed can you close out the batch processing. Lets begin by following the steps below.

Note

Each of these steps might take anywhere from a few minutes to multiple hours.

Cleaning

Click the pencil icon on the top right corner to edit the fields in this step.

Each toggle switch must be enabled before moving forwards to the next tab (Demineralizing)

Toggles must be enabled for the following processes: - Label Tubes and Caps - Remove all visible signs of surface contamination using rotary tool - Rinse samples w/dH20 - Sonicate samples with dH20 - cycle 1 - Sonicate samples with dH20 - cycle 2 - Sonicate samples with 95% ethanol - Sonicate samples with 100% ethanol - Dry samples in oven

Click Save

After the toggles have been enabled and saved, you will see "Start Date" for Sonicate samples with dH20 - cycle 1, Sonicate samples with 95% ethanol, Dry samples in oven, Sonicate samples with dH20 - cycle 2, Removed cooled samples from tubes, weigh, and return.

Note: Dates cannot be edited, the dates are taken from when the toggle switch was enabled.

After you save the above one new field will appear, enable this toggle once completed and then click save

  • Remove cooled samples from tubes, weigh, and return

Click Save

If you are not able to go to the next tab, please check and ensure all fields and toggles are filled out properly

Demineralizing

Click the pencil icon on the top right corner to edit the fields in this step.

Each toggle switch must be enabled before moving forwards to the next tab (Remove Humic Acid)

Toggle must be enabled for: - Treat samples with 40ml 0.25 M hydrochloric acid until spongy. Change acid every 2-4 days. Treat until there are no hard spots; test with dental pick. Discard waste appropriately.

click Save

Two new fields will appear, click the pencil icon again to edit to enable the toggle switches

Toggles must be enabled for: - Treatment of samples with 40ml 0.25 M hydrochloric acid complete

Note: Dates cannot be edited, the dates are taken from when the toggle switch was enabled.

Click Save

One new field will appear, click the pencil icon again to edit to enable the toggle switch

Toggles must be enabled for: - Rinse demineralized samples 3x with dH20

Click Save

You will be moved to the next tab, "Remove Humic Acid", if you are not able to go to the next tab, please check and ensure all fields and toggles are filled out properly

Remove Humic Acid

Click the pencil icon on the top right corner to edit the fields in this step.

Each toggle switch must be enabled before moving forwards to the next tab (Solubilizing).

Toggles must be enabled for:

  • Treat samples with 40ml 0.125 M sodium hydroxide for 24 hrs. Discard waste appropriately. Rinse samples 5x with dH20.
  • Rinse 1 Start
  • Rinse 1 End
  • Rinse 2 Start
  • Rinse 2 End
  • Rinse 3 Start
  • Rinse 3 End
  • Rinse 4 Start
  • Rinse 4 End
  • Rinse 5 Start
  • Rinse 5 End

Click Save

One new field will appear, click the pencil icon again to edit to enable the toggle switches.

Note: Dates cannot be edited, the dates are taken from when the toggle switch was enabled.

  • Treatment and Rinse Completed

Click Save

You will be moved to the next tab, "Solubilizing", if you are not able to go to the next tab, please ensure all fields and toggles are filled out properly

Solubilizing

Click the pencil icon on the top right corner to edit the fields in this step.

Each toggle switch must be enabled before moving forwards to the next tab (Freeze Drying).

Toggle must be enabled and fields entered for:

  • Clean vials and lids. Label.
  • Add 15ml pH 3 dH20 to samples and cover tubes with double layer of plastic wrap. Cap tightly.
  • Place capped tubes in oven for 24 hours.
  • Centrifuge tubes and transfer supernatant into PTFE beakers. Place beakers in oven to evaporate
  • Number of Acid-Heat Treatments
  • Number of Collagen Transfers
  • Close vials and freeze until solid. Tilting vials while freezing can help prevent cracking.

Click Save

You will be moved to the next tab, "Freeze Drying", if you are not able to go to the next tab, please ensure all fields and toggles are filled out properly.

Freeze Drying

Click the pencil icon on the top right corner to edit the fields in this step.

Each toggle switch must be enabled before moving forwards to the next tab (Collagen Yield).

Toggles must be enabled for:

  • Turn on freeze-dryer. Replace vial lids with Kimwipe caps. Ensure samples are frozen solid.
  • Freeze-dry samples - this may take 1 - 3 days. Once collagen is dry, cap vials tightly.
  • Freeze-dry samples step completed

Click Save

Note: Dates cannot be edited, the dates are taken from when the toggle switch was enabled.

You will be moved to the next tab, "Collagen Yield", if you are not able to go to the next tab, please ensure all fields and toggles are filled out properly.

Collagen Yield

Click the pencil icon on the top right corner to edit the fields in this step.

Each toggle switch must be enabled before the Isotope Batch can be saved.

Toggle must be enabled for:

  • Weigh dry samples in vials with lids and record combined weight.

Click Save

You should see "Result Status" field on the top showing that it is Completed.

Isotope Batch Fields

The screen fields are shown below. Fields in bold are required.

Tip

dH2O is Distilled Water

Attributes Data Type Size Validation Rule Required/Optional
Lab Id id Can choose from the option available Required
External Case # Alphanumeric 32 chars Can only contain letters and numerals Optional
Isotope Batch Number Alphanumeric 32 chars Can only contain letters and numerals Required
Status String 32 chars valid statuses are Open, Associating Isotopes, Cleaning, Demineralizing, Removal Humic Acids, Solubilizing, Freeze Drying Collagen, Determining Collagen Yield and closed Required
Cleaning start date Date Required
Label tubes and caps Boolean true/false toggle Required
Remove all visible signs of surface contamination Boolean true/false toggle Required
Rinse sample with dH2O Boolean true/false toggle Required
Sonicate samples with dH2O-cycle1 Boolean true/false toggle Required
Sonicate samples with dH2O-cycle1 Start Date Date Required
Sonicate samples with dH2O-cycle2 Boolean true/false toggle Required
Sonicate samples with dH2O-cycle2 Start Date Date Required
Sonicate samples with 95% ethanol Boolean true/false toggle Required
Sonicate samples with 95% ethanol Start Date Date Required
Sonicate samples with 100% ethanol Boolean true/false toggle Required
Sonicate samples with 100% ethanol Start Date Date Required
Dry samples start Boolean true/false toggle Required
Dry sample start date Date Required
Dry samples end Boolean true/false toggle Required
Dry sample end date Date Required
Cleaning initials Alphanumeric 32 chars Can only contain letters and numerals Required
Demineralizing Treatment Start Boolean true/false toggle Required
Demineralizing Treatment End Boolean true/false toggle Required
Demineralizing Treatment Start Date Date Required
Demineralizing Treatment End Date Date Required
Rinse Demineralized Samples Boolean true/false toggle Required
Removal Humic Acids treatment start Boolean true/false toggle Required
Removal Humic Acids treatment End Boolean true/false toggle Required
Removal Humic Acids treatment start Date Date Required
Removal Humic Acids treatment End Date Date Required
Removal Humic Acids treatment rinse 1 start Boolean true/false toggle Required
Removal Humic Acids treatment rinse 1 End Boolean true/false toggle Required
Removal Humic Acids treatment rinse 1 start Date Date Required
Removal Humic Acids treatment rinse 1 End Date Date Required
Removal Humic Acids treatment rinse 2 start Boolean true/false toggle Required
Removal Humic Acids treatment rinse 2 End Boolean true/false toggle Required
Removal Humic Acids treatment rinse 2 start Date Date Required
Removal Humic Acids treatment rinse 2 End Date Date Required
Removal Humic Acids treatment rinse 3 start Boolean true/false toggle Required
Removal Humic Acids treatment rinse 3 End Boolean true/false toggle Required
Removal Humic Acids treatment rinse 3 start Date Date Required
Removal Humic Acids treatment rinse 3 End Date Date Required
Removal Humic Acids treatment rinse 4 start Boolean true/false toggle Required
Removal Humic Acids treatment rinse 4 End Boolean true/false toggle Required
Removal Humic Acids treatment rinse 4 start Date Date Required
Removal Humic Acids treatment rinse 4 End Date Date Required
Removal Humic Acids treatment rinse 5 start Boolean true/false toggle Required
Removal Humic Acids treatment rinse 5 End Boolean true/false toggle Required
Removal Humic Acids treatment rinse 5 start Date Date Required
Removal Humic Acids treatment rinse 5 End Date Date Required
Solubilizing clean vials and lids Boolean true/false toggle Required
Solubilizing clean vials and lids date Date Required
Solubilizing add soluble Boolean true/false toggle Required
Solubilizing place in oven Boolean true/false toggle Required
Solubilizing centrifuge tubes Boolean true/false toggle Required
Solubilizing num acid heat treatment Number 32 chars numeric Required
Solubilizing num collagen transfers Number 32 chars numeric Required
Solubilizing freeze vials Boolean true/false toggle Required
Solubilizing freeze vials date Date Required
Freeze Drying Collagen on Boolean true/false toggle Required
Freeze Drying Collagen Start Boolean true/false toggle Required
Freeze Drying Collagen End Boolean true/false toggle Required
Freeze Drying Collagen Start Date Date Required
Freeze Drying Collagen End Date Date must be greater than start date Required
Combined Sample Weight Boolean true/false toggle Optional
Notes String 256 chars Can contains a string alphanumeric values Optional

Last update: 2024-05-05
Created: 2023-12-23